Stefano Vassanelli's 'NeuroChip' Lab

People

Prof. Stefano Vassanelli
Team Leader
NeuroChip Laboratory
Department of Biomedical Sciences
University of Padova
Via Francesco Marzolo 3
Padova - 35131, Italy
E-Mail: stefano.vassanelli@unipd.it

About Professor Stefano Vassanelli    

    Stefano Vassanelli was born in Padova, Italy, in 16-06-1968 and received the degree cum Laude in Medicine and Surgery at the University of Padova in 1992.

    For his doctoral thesis he obtained in 1992 the “Casati” price awarded from the “Accademia Nationale dei Lincei”.

    In 1993 he spent one year as Post Docr at the Oregon Graduate Institute of Science and Technology, Dpt. of Biochemistry, (Portland. Oregon, USA), in the laboratory of Prof. K.D.Garlid.

    There, he contributed to clarify the working mechanism of the uncoupling protein (UCP-1), which is involved in the thermogenesis by mitochondria of the brown adipose tissue.

    In 1994 he started a PhD in Molecular and Cell Biology and Pathology at the Dept. of Biomedical Sciences of the University of Padova.

    From 1995 to 2000, he was Post Doc at the Max-Planck Institute for Biochemistry, Dpt. Membrane and Neurophysics (Martinsried, Germany) directed by Prof. P.Fromherz, working on the first recording of rat neurons by semiconductor-based field-effect-transistors.

    In 1999 he obtained the PhD in Molecular and Cell Biology and Pathology.

    In 2002 he won the second price in the StartCup competition for high technology applied research organized by the Universities of Padova and Bologna.

    Since 2000 he is leading a research group at the University of Padova, Dpt. of Human Anatomy and Physiology, section of Physiology.

    The current research activity of Prof. Vassanelli is focused on the development of new biotechnologies based on the application of microelectronics to biology and medicine.

    Silicon microchips are interfaced with mammalian neurons, muscle cells or transfected cell lines for the characterization of ion channels and the assessment of their involvement in human pathologies.

    Here, field effect transistors and capacitors integrated in the microchip are used to monitor and control ions displacements between cell and semiconductor.

    Silicon microchips are also used for introducing molecules such as drugs or genetic constructs into single cultured cells.

    Implantable chips are developed for recording and stimulation of neuronal networks “in vivo”. These techniques offer promising applications for basic research in life science, for high throughput screenings directed to identify drugs and genes functions and for neuroprosthetics.

    Other activities in collaboration with other groups include the effect of electromagentic fields on ion channels and neurons and the role of anti-GAD antibodies in the pathogenesis of epilepsy.

Patents    

    2. Mahmud M, Vassanelli S (2012),  SigMate: A Comprehensive Software Package for Processing and Analysis of Neuronal Signals, patent filed with the Italian Patent Authority (application no. 001963109).

    1. Vassanelli S, Cellere G, Bandiera L, Borgo M (2005),  Biochip Electroporator and its use in multi-site, single-cell electroporation, PCT/EP2004/000789; PCT Pubbl. n. WO2005075656.

Selected Publications    

    Book Chapters

    2011

    1. Mahmud M, Bertoldo A, Vassanelli S, "EEG Based Brain Machine Interfacing: Navigation of Mobile Robotic Device,"In: Janusz Bedkwoski (ed.) Mobile Robots - Control Architectures, Bio-interfacing, Navigation, Multi Robot Motion Planning and Operator Training, Intech: Rijeka, Croatia, ISBN: 978-953-307-842-7, pp. 129-144.

    Journal Publications

    2012

    21. Mahmud M, Travalin D, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "An Automated Classification Method for Single Sweep Local Field Potentials Recorded from Rat Barrel Cortex under Mechanical Whisker Stimulation," Journal of Medical and Biological Engineering, doi: 10.5405/jmbe.923 (in press).

    20. Mahmud M, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "SigMate: A Matlab-based automated tool for extracellular neuronal signal processing and analysis," Journal of Neuroscience Methods, Vol. 207, No. 1, 2012, pp. 97-112, doi: 10.1016/j.jneumeth.2012.03.009.

    19. Vassanelli S, Mahmud M, Girardi S, Maschietto M, "On the Way to Large-Scale and High-Resolution Brain-Chip Interfacing," Cognitive Computation, Vol. 4, No. 1, 2012, pp. 71-81, doi: 10.1007/s12559-011-9121-4.

    18. Cianci E, Lattanzio S, Seguini G, Vassanelli S, Fanciulli M, "Atomic layer deposited TiO2 for implantable brain-chip interfacing devices," Thin Solid Films, Vol. 520, 2012, pp. 4745-4748, doi: 10.1016/j.tsf.2011.10.197.

    2011

    17. Quarta M, Scorzeto M, Canato M, Dal Maschio M, Conte D, Blaauw B, Vassanelli S, Reggiani C, "The modulation of myogenic cells differentiation using a semiconductor-muscle junction," Biomaterials, Vol. 32, 2011, pp. 4228-4237.

    16. Giacomello M, Girardi S, Scorzeto M, Peruffo A, Maschietto M, Cozzi B, Vassanelli S, "Stimulation of Ca2+ Signals in neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors," Journal of Neuroscience Methods, Vol. 198, No. 1, 2011, pp. 1-7.

    15. Mahmud M, Pasqualotto E, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "An Automated Method for the Detection of Layer Activation Order in Information Processing Pathways of Rat Barrel Cortex under Mechanical Whisker Stimulation," Journal of Neuroscience Methods, Vol. 196, No. 1, March 2011, pp. 141-150.

    2008

    14. Vassanelli S, Bandiera L, Borgo M, Cellere G, Santoni L, Bersani C, Salamon M, Zaccolo M, Lorenzelli L, Girardi S, Maschietto M, Dal Maschio M, Paccagnella A, "Space and time-resolved gene expression experiments on cultured mammalian cells by a single-cell electroporation microarray,"  New Biotechnology, Volume 25, Issue 1, June 2008, Pages 55-67, ISSN 1871-6784.

    13. Vianello M, Bisson G, Dal Maschio M, Vassanelli S, Girardi S, Muciagnat C, Fountzoulas K, Giometto B, "Increased spontaneous activity of a network of hippocampal neurons in culture caused by suppression of inhibitory potentials mediated by anti-gad antibodies," Autoimmunity (41): 66-73.

    12. Bisson G, Balduzzo M, Minelli AT, Dal Maschio M, Vassanelli S, "Epileptiform Activity in Hippocampal Neuronal Network Triggered by Human Anti-GAD Antibodies," Chaos and Complexity Letters, vol. 4, no. 2, pp. 257-275, SSN: 1054-1500 3 2008.

    11. Medlam L, Maschietto M, Minelli T, Boccaccio P, Moschini G, Vassanelli S, "Increased and Persistent Potassium Conductance in CHO Transfected Cells Following Exposure to Low-Frequency and Low-Intensity Magnetic Field," LNL- Annual Report, p. 78-79, ISSN: 1828-85454 2008.

    2007

    10. Martelli L, Ragazzi E, Di Mario F, Martelli M, Castagliuolo I, Dal Maschio M, Palu' G, Maschietto M, Scorzeto M, Vassanelli S, Brun P, "A potential role for the vanilloid receptor TRPV1 in the therapeutic effect of curcumin in dinitrobenzene sulphonic acid-induced colitis in mice" Neurogastroenterology and Motility (19): 668-674.

    2006

    9. Vianello M, Giometto B, Vassanelli S, Canato M, Betterle C, Mucignat C, "Peculiar labeling of cultured hippocampal neurons by different sera harboring anti-glutamic acid decarboxylase autoantibodies (GAD-Ab)," Experimental Neurology (202): 514-518.

    2005

    8. Kiessling V, Vassanelli S, "Potassium channel gating in adhesion: from an oocyte-silicon to a neuron-astrocyte adhesion contact," European Biophysics Journal with Biophysics Letters  (34): 113-26.

    2003

    7. Naro F, De Arcangelis V, Coletti D, Molinaro M, Zani B, Vassanelli S, Reggiani C, Teti A, Adamo S, "Increase in cytosolic Ca2+ induced by elevation of extracellular Ca2+ in skeletal myogenic cells," American Journal of Physiology - Cell Physiology (284): C969-76.

    2001

    6. Antonucci DE, Lim ST, Vassanelli S, Trimmer JS, "Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons,"  Neuroscience  (108): 69-81.

    1999

    5. Vassanelli S, Fromherz P, "Transistor probes local potassium conductances in the adhesion region of cultured rat hippocampal neurons,"  Journal of Neuroscience (19): 6767-6773.

    1998

    4. Vassanelli S, Fromherz P, "Transistor-records of excitable neurons from rat brain,"  Applied Physics A - Materials Science & Processing   (66): 459-463.

    1997

    3. Vassanelli S, Fromherz P, "Neurons from rat brain coupled to transistors,"  Applied Physics A - Materials Science & Processing   (65): 85-88.

    1996

    2. Garlid KD, Orosz DE, Modriansky M, Vassanelli S, Jesek P, "On the Mechanism of Fatty Acid-induced Proton Transport by Mitochondrial Uncoupling Protein,"  The Journal of Biological Chemistry, vol. 271; p. 2615-2620, ISSN: 0021-9258.

    1992

    1. Bernardi P, Vassanelli S, Veronese P, Colonna R, Szabò I, Zoratti M, "Modulation of the Mitochondrial Transition Pore,"  The Journal of Biological Chemistry, vol. 267; p. 2934-2939, ISSN: 0021-9258.

    Peer-Reviewed Conference Publications

    2012

    25. Mahmud M, Rahman MM, Vassanelli S, "Realistic Neuromuscular Junction Candidate Model for Neuroprosthetic Interface," Accepted at:  2012 ASME Dynamic Systems and Control Conference (DSCC2012), Florida, USA, Oct. 17-19, 201.

    24. Vassanelli S, Felderer F, Mahmud M, Maschietto M, Girardi S, "CyberRat Probes: High-Resolution Biohybrid Devices for Probing the Brain," In: T.J. Prescott et al. (Eds.): Living Machines 2012, LNAI 7375, pp. 274–285, 2012.

    23. Mahmud M, Travalin D, Girardi S, Maschietto M, Felderer F, Vassanelli S, "Single LFP Sorting for High-resolution Brain-chip Interfacing,"In: H. Zhang et al. (Eds.): BICS 2012, LNAI 7366, pp. 329–337, 2012.

    22. Mahmud M, Girardi S, Maschietto M, Vassanelli S, "An Automated Method to Remove Artifacts Induced by Microstimulation in Local Field Potentials Recorded from Rat Somatosensory Cortex," Accepted at: The 3rd IEEE ISSNIP Biosignals and Biorobotics conference (BRC2012), Manaus, Brazil, January 9-12, 2012.

    21. Mahmud M, Girardi S, Maschietto M, Vassanelli S, "A MATLAB Based Tool for Cortical Layer Activation Order Detection through Latency Calculation in Local Field Potentials Recorded from Rat Barrel Cortex by Brain-Chip Interface," Accepted at:  The 3rd IEEE ISSNIP Biosignals and Biorobotics conference (BRC2012), Manaus, Brazil, January 9-12, 2012.

    2011

    20. Mahmud M, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "SigMate: An Automated Comprehensive Software Tool for Analysis of Neuronal Signals," In:  Proceedings of the Australasian Cognitive Neuroscience Conference 2011 (ACNC2011), Sydney, Australia, December 9-12, 2011.

    19. Rahman MM, Mahmud M, Vassanelli S, Bistability of Sodium channels increases the safety-factor at the Neuromuscular Junction," In:  Proceedings of the Australasian Cognitive Neuroscience Conference 2011 (ACNC2011), Sydney, Australia, December 9-12, 2011.

    18. Rahman MM, Mahmud M, Vassanelli S, Sodium Channels' Kinetics under Self-gating Condition at Neuromuscular Junction," In:  Proceedings of the 4th International Conference on BioMedical Engineering and Informatics (BMEI2011), Shanghai, China, October 15-17, 2011, pp. 990-994.

    17. Vassanelli S, "Brain-Chip Interfacing: The Present and The Future," In: FET11 Essence, The European Future Technologies Conference and Exhibition, 4-6 May, Budapest, Hungary, 2011.

    16. Mahmud M, Girardi S, Maschietto M, Pasqualotto E, Vassanelli S, "An Automated Method to Determine Angular Preferentiality using LFPs Recorded from Rat Barrel Cortex by Brain-Chip Interface under Mechanical Whisker Stimulation," In: Proceedings of the 33rd Annual International Conference of IEEE EMBS (IEEE-EMBC2011), Boston, USA, August 30 - September 3, 2011, pp. 2307-2310.

    15. Rahman MM, Mahmud M, Vassanelli S, "Effect of Self-Gating on Action Potential Firing at Neuromuscular Junction," In: Proceedings of the 33rd Annual International Conference of IEEE EMBS (IEEE-EMBC2011), Boston, USA, August 30 - September 3, 2011, pp. 4082-4085.

    14. Mahmud M, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "A MATLAB Based Toolbox for Processing and Analysis of Multichannel, Multisource Cortical Local Field Potential," In: Front. Neuroinform. Conference Abstract: 4th INCF Congress of Neuroinformatics, Boston, USA, September 4-6, 2011, doi: 10.3389/conf.fninf.2011.08.00148.

    13. Rahman MM, Mahmud M, Vassanelli S, "Self-Gating Effect at the Rat Neuromuscular Junction," In: Front. Neuroinform. Conference Abstract: 4th INCF Congress of Neuroinformatics, Boston, USA, September 4-6, 2011, doi: 10.3389/conf.fninf.2011.08.00018.

    12. Mahmud M, Bertoldo A, Girardi S, Maschietto M, Pasqualotto E, Vassanelli S, "SigMate: A Comprehensive Software Package for Extracellular Neuronal Signal Processing and Analysis," In:  Proceedings of the 5th International IEEE EMBS Conference on Neural Engineering (IEEE EMBS-NER2011), Cancun, Maxico, April 27 - May 1, 2011, pp. 88-91.

    11. Rahman M M, Mahmud M, Vassanelli S, "Self-Gating of Sodium Channels at Neuromuscular Junction," In:  Proceedings of the 5th International IEEE EMBS Conference on Neural Engineering (IEEE EMBS-NER2011), Cancun, Maxico, April 27 - May 1, 2011, pp. 208-211.

    10. Girardi S, Maschietto M, Zeitler R, Mahmud M, Vassanelli S, "High Resolution Cortical Imaging Using Electrolyte-(Metal)-Oxide-Semiconductor Field Effect Transistors," In:  Proceedings of the 5th International IEEE EMBS Conference on Neural Engineering (IEEE EMBS-NER2011), Cancun, Maxico, April 27 - May 1, 2011, pp. 269-272.

    9. Mahmud M, Travalin D, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "An Automated Method for Clustering Single Sweep Local Field Potentials Recorded from Rat Barrel Cortex," In: Proceedings of the ISSNIP Biosignals and Biorobotics Conference 2011 (BRC2011), Vitoria, Brazil, 6-8 January 2011, pp. 1-5.

    2010

    8. Mahmud M, Travalin D, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "A Contour Based Automatic Method to Classify Local Field Potentials Recorded from Rat Barrel Cortex," In: Proceedings of the 5th Cairo International Conference on Biomedical Engineering (CIBEC2010), Cairo, Egypt, 16-18 December 2010, pp. 163-166.

    7. Mahmud M, Bertoldo A, Girardi S, Maschietto M, Vassanelli S, "SigMate: A MATLAB-based Neuronal Signal Processing Tool," In: Proceedings of the 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE EMBC2010), Buenos Aires, Argentina, September 2010, pp. 1352-1355.

    6. Mahmud M, Bertoldo A, Maschietto M, Girardi S, Vassanelli S, "Automatic Detection of Layer Activation Order in Information Processing Pathways of Rat Barrel Cortex under Mechanical Whisker Stimulation," In: Proceedings of the 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE EMBC2010), Buenos Aires, Argentina, September 2010, pp. 6095-6098.

    5. Mahmud M, Girardi S, Maschietto M, Bertoldo A, Vassanelli S, "Processing of Neuronal Signals Recorded by Brain-Chip Interface from Surface of the S1 Brain Cortex," In: Proceedings of the 36th Annual Northeast Bioengineering Conference 2010 (NEBEC2010), New York, USA, 25-28 March 2010, pp. 1-2.

    2009

    4. Mahmud M, Girardi S, Maschietto M, Rahman MM, Bertoldo A, Vassanelli S, "Noise Characterization of Electrophysiological Signals Recorded from High Resolution Brain-Chip Interface," In: Proceedings of the International Symposium on Bioelectronics and Bioinformatics (ISBB2009), Melbourne, Australia, 9-11 December 2009, pp. 84-87.

    3. Mahmud M, Girardi S, Maschietto M, Rahman MM, Bertoldo A, Vassanelli S, "Slow Stimulus Artifact Removal through Peak-Valley Detection of Neuronal Signals Recorded from Somatosensory Cortex by High Resolution Brain-Chip Interface," In: IFMBE Proceedings of the World Congress 2009 in Medical Physics and Biomedical Engineering (WC2009). Munich, Germany, 7-12 September 2009, vol. 25/IV, pp. 2062-2065.

    2. Maschietteo M, Girardi S, Dal Maschio M, Vassanelli S, "Microchip-Integrated EOSCs (Electrolyte Oxide Semiconductor Capacitors) as Devices for High Efficiency and Selective Electroporation of Mammalian Cells," In: IFMBE Proceedings of the World Congress 2009 in Medical Physics and Biomedical Engineering (WC2009). Munich, Germany, 7-12 September 2009, vol. 25/VIII, pp. 321–324.

    1. Maschietto M, Mahmud M, Girardi S, Vassanelli S, "A High Resolution Bi-Directional Communication through a Brain-Chip Interface," In: Proceedings of the 2009 ECSIS Symposium on Advaced Technologies for Enhanced Quality of Life (AT-EQUAL 2009), Iasi, Romania, July 22 - 24, pp. 32-35.

    Other Publications

    5. Maschietto M, Girardi S, Felderer F, Pasqualotto E, Mahmud M, Vassanelli S, "Microchip-Integrated Electrolyte Oxide Semiconductor Field Effect Transistors (EOSFETs) as Devices for High Resolution Recordings in Rat Brain Cortex," Poster at the 8th International Brain Research Organization World Congress of Neuroscience (IBRO2011) at Florence, Italy, July 14-18, 2011.

    4. Ferrario A, Scaramuzza M, Pasqualotto E, De Toni A, Paccagnella A, Maschietto M, Vassanelli S, "Electrochemical impedance spectroscopy study of the cells adhesion over microelectrodes array," 2011 7th Conference on Ph.D. Research in Microelectronics and Electronics (PRIME), pp.57-60, July 3-7, 2011, Madonna di Campiglio, Trento – Italy.

    3. Pasqualotto E, Girardi S, Maschietto M, Mahmud M, Vassanelli S, "CyberRat: A Brain-Chip Interface for High-Resolution Bi-directional Communication," Poster at the Information and Communication Technologies Conference (ICT'10) at Brussels, Belgium, 27-29 September, 2010.

    2. Girardi S, Maschietto M, Mahmud M, Vassanelli S, "CyberRat: A High Resolution Bi-Directional Communication through a Brain-Chip Interface," Poster at the European Future Technologies Conference (FET'09) at Prague, Czeck Republic, 21-23 April, 2009.

    1. Maschietto M, Girardi S, Vassanelli S, "Rat brain Nav1.2 channel sorting: auxiliary subunits/ECM proteins interaction in cultured neurons," Eur. Biophys. J. Biophys. Lett. 34(6): 647. P-262. 15th IUPAB & 5th EBSA International Biophysics Congress.